Mupirocin Calcium EP impurity F is a calcium salt derivative of the cyclic depsipeptide mupirocin, characterized by a truncated macrocyclic lactone ring and a modified side chain. It retains the core 2-pyrrolidinone motif but exhibits a linearized ester linkage at the C-terminus, replacing the parent drug's cyclic structure. This impurity arises via partial hydrolysis of the native lactone ring during synthesis or storage. Its distinct functional groups include a free carboxylic acid and a calcium-coordinated hydroxyl moiety, conferring altered solubility and chromatographic behavior. This compound serves as a critical HPLC reference standard for quantifying process-related impurities in mupirocin active pharmaceutical ingredients.
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InChI=1S/C24H40O9/c1-14(11-21(28)31-9-7-5-4-6-8-20(26)27)10-18-23(30)22(29)17(13-32-18)12-19-24(33-19)15(2)16(3)25/h11,15-19,22-25,29-30H,4-10,12-13H2,1-3H3,(H,26,27)/b14-11+/t15-,16-,17-,18-,19-,22+,23-,24-/m0/s1
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