Prednisolone 21-formate is a corticosteroid derivative featuring a formate ester substitution at the C21 hydroxyl position of the parent compound, prednisolone. The molecule retains the characteristic oxidized A-ring (ฮยน(ยฒ) double bond), 11ฮฒ-hydroxyl, and 17ฮฑ-hydroxyl functionalities. The 21-formate ester introduces a labile acyloxy group, rendering it a potential synthetic byproduct during esterification processes or a degradation product under acidic conditions. This compound serves as an HPLC reference standard for quantifying 21-ester-related impurities in prednisolone API batches.
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InChI=1S/C22H28O6/c1-20-7-5-14(24)9-13(20)3-4-15-16-6-8-22(27,18(26)11-28-12-23)21(16,2)10-17(25)19(15)20/h5,7,9,12,15-17,19,25,27H,3-4,6,8,10-11H2,1-2H3/t15-,16-,17-,19+,20-,21-,22-/m0/s1
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